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Titolo: Radiochemical assay for determination of dihydropyrimidinase activity using reversed-phase high-performance liquid chromatography
Autore: Van Kuilenburg, ABP; Van Lenthe, H; Van Gennip, AH;
- Indirizzi:
- Univtherlandsm, Acad Med Ctr, Lab Genet Metab Dis, NL-1105 AZ Amsterdam, Ne Univ Amsterdam Amsterdam Netherlands NL-1105 AZ NL-1105 AZ Amsterdam, Ne Univtherlandsm, Acad Med Ctr, Emma Childrens Hosp, NL-1100 DE Amsterdam, Ne Univ Amsterdam Amsterdam Netherlands NL-1100 DE NL-1100 DE Amsterdam, Ne Dept Clin Chem, NL-1100 DE Amsterdam, Netherlands Dept Clin Chem Amsterdam Netherlands NL-1100 DE E Amsterdam, Netherlands
- Titolo Testata:
- JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2,
volume: 729,
anno: 1999,
pagine: 307 - 314
- SICI:
- 1387-2273(19990611)729:1-2<307:RAFDOD>2.0.ZU;2-T
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- 5-FLUOROURACIL RELATED TOXICITY; SPLICE DONOR SITE; POINT MUTATION; PYRIMIDINE DEGRADATION; RAT-LIVER; DEHYDROGENASE; HETEROZYGOSITY; PURIFICATION; DEFICIENCY; ACID;
- Keywords:
- enzymes; dihydropyrimidinase;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Life Sciences
- Citazioni:
- 23
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Van Kuilenburg, ABP UnivL-1105rdam, Acad Med Ctr, Lab Genet Metab Dis, Meibergdreef 9,FO-224, N Univ Amsterdam Meibergdreef 9,FO-224 Amsterdam Netherlands NL-1105 AZ
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- Citazione:
- A.B.P. Van Kuilenburg et al., "Radiochemical assay for determination of dihydropyrimidinase activity using reversed-phase high-performance liquid chromatography", J CHROMAT B, 729(1-2), 1999, pp. 307-314
Abstract
A radiochemical assay was developed to measure the activity of dihydropyrimidinase (DHP) in human liver homogenates. The method is based on the separation of radiolabeled dihydrouracil from N-carbamyl-beta-alanine by HPLC with on-line detection of radioactivity combined with detection of (CO2)-C-14by liquid scintillation counting. The assay was linear with time and protein concentration. The minimum amount of radiolabeled products which could be determined proved to be 12 pmol using a purified stock solution of [2-C-14]-5,6-dihydrouracil. This highly sensitive assay is especially suitable toidentify patients with a dihydropyrimidinase deficiency. (C) 1999 ElsevierScience B.V. All rights reserved.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/05/13 alle ore 20:24:33