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Titolo:
BIOCHEMICAL-EVIDENCE OF FUNCTIONAL INTERACTION BETWEEN MU-OPIOID AND DELTA-OPIOID RECEPTORS IN SK-N-BE NEUROBLASTOMA CELL-LINE
Autore:
PALAZZI E; CEPPI E; GUGLIELMETTI F; CATOZZI L; AMOROSO D; GROPPETTI A;
Indirizzi:
UNIV MILAN,DEPT MED PHARMACOL EMILIO TRABUCCHI,VIA VANVITELLI 32 I-20129 MILAN ITALY
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 67, anno: 1996,
pagine: 138 - 144
SICI:
0022-3042(1996)67:1<138:BOFIBM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENYLATE-CYCLASE ACTIVITY; RAT-BRAIN HOMOGENATE; PHARMACOLOGICAL CHARACTERIZATION; OPIATE RECEPTOR; MORPHINE-LIKE; BINDING; CLONING; EXPRESSION; STIMULATION; ENKEPHALIN;
Keywords:
NEUROBLASTOMA CELL LINES; MU- AND DELTA-OPIOID RECEPTORS; FUNCTIONAL INTERACTION; ADENYLYL CYCLASE ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
E. Palazzi et al., "BIOCHEMICAL-EVIDENCE OF FUNCTIONAL INTERACTION BETWEEN MU-OPIOID AND DELTA-OPIOID RECEPTORS IN SK-N-BE NEUROBLASTOMA CELL-LINE", Journal of neurochemistry, 67(1), 1996, pp. 138-144

Abstract

Radioligand binding assays and functional experiments revealed that the SK-N-BE neuroblastoma cell line expresses a similar ratio of mu- and delta-opioid receptors, both negatively coupled to adenylyl cyclase through pertussis toxin-sensitive G proteins. Our Findings also indicate that some functional interaction occurred between the two opioid subtypes; in fact, long-term exposure to [D-Ala(2)-N-methyl-Phe(4)-Gly-ol(5)] enkephalin (DAMGO), a mu-selective agonist, sensitized the functional response of the delta-selective agonist but not vice versa, It is interesting that in acute interaction experiments, we observed a shift to the fight of the concentration-effect curve of either DAMGO or [D-Pen(2,5)]enkephalin (DPDPE), a delta-selective agonist, as a result of DPDPE or DAMGO administration, respectively, In addition, low dosesof naloxone, an antagonist selective for mu receptors, increased the inhibitory effect of [D-Ala(2),D-Met(5)] enkephalinamide (DAME), a mixed mu/delta agonist, on adenylyl cyclase activity. Taken overall, these data support the hypothesis of the existence of a cross talk betweenmu and delta receptors in the SK-N-BE cell line.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/17 alle ore 11:08:40