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Titolo:
THE HSP60 GENE OF THE HUMAN PATHOGENIC FUNGUS COCCIDIOIDES-IMMITIS ENCODES A T-CELL REACTIVE PROTEIN
Autore:
THOMAS PW; WYCKOFF EE; PISHKO EJ; YU JJ; KIRKLAND TN; COLE GT;
Indirizzi:
MED COLL OHIO,DEPT IMMUNOL & MICROBIOL,3050 ARLINGTON AVE TOLEDO OH 43614 MED COLL OHIO,DEPT IMMUNOL & MICROBIOL TOLEDO OH 43614 UNIV TEXAS,DEPT MICROBIOL AUSTIN TX 78712 BOSTON COLL,MERKERT CHEM CTR CHESTNUT HILL MA 02167 UNIV CALIF SAN DIEGO,VET AFFAIRS MED CTR,DEPT PATHOL SAN DIEGO CA 92161 UNIV CALIF SAN DIEGO,VET AFFAIRS MED CTR,DEPT MED SAN DIEGO CA 92161
Titolo Testata:
Gene
fascicolo: 1-2, volume: 199, anno: 1997,
pagine: 83 - 91
SICI:
0378-1119(1997)199:1-2<83:THGOTH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
WALL FRACTION; EXPRESSION; SEQUENCES; ANTIGEN; CLONING; FAMILY; ALIGNMENT; BACTERIAL; PLANT;
Keywords:
FUNGAL RESPIRATORY PATHOGEN; GENOMIC/CDNA SEQUENCES; SOUTHERN/NORTHERN HYBRIDIZATION; PROTEIN EXPRESSION/IMMUNOLOCALIZATION; PROTEIN HOMOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
P.W. Thomas et al., "THE HSP60 GENE OF THE HUMAN PATHOGENIC FUNGUS COCCIDIOIDES-IMMITIS ENCODES A T-CELL REACTIVE PROTEIN", Gene, 199(1-2), 1997, pp. 83-91

Abstract

A heat shock protein-encoding gene (hsp60) from the human respiratoryfungal pathogen, Coccidioides immitis (Ci), was cloned, sequenced, chromosome-mapped, expressed and immunolocalized in parasitic cells. Both the genomic and cDNA sequences are presented. The transcription start point and poly (A) addition site were confirmed. The hsp60 gene contains two introns and a 1782-bp ORF which translates a 594-amino acid (aa) protein of 62.4 kDa and pI of 5.6. The translated protein revealedtwo potential N-glycosylation sites. The deduced HSP60 showed 78-83% aa sequence similarity to reported fungal HSP60 proteins. The hsp60 gene was mapped to chromosome III of Ci and was shown to be a single copy gene by Southern and Northern hybridization. Expression of a 1737-bpcDNA fragment of the hsp60 gene in E. coli resulted in production of a recombinant protein. Amino acid sequence analysis of the recombinantprotein confirmed that it was encoded by the Ci hsp60 gene. Antiserumraised in mice against the isolated recombinant protein immunolocalized HSP60 in the cytoplasm and wall of parasitic cells of Ci. The recombinant HSP60 was used to immunize BALB/c mice and was shown to induce proliferation of T cells isolated from lymph nodes of these animals. The hsp60 gene of Ci is the first reported heat-shock protein gene of this human pathogen. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/06/18 alle ore 17:30:59