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Titolo:
ISOLATION AND CHARACTERIZATION OF THE UREASE GENE (URE) FROM THE PATHOGENIC FUNGUS COCCIDIOIDES-IMMITIS
Autore:
YU JJ; SMITHSON SL; THOMAS PW; KIRKLAND TN; COLE GT;
Indirizzi:
MED COLL OHIO,DEPT IMMUNOL & MICROBIOL,3000 ARLINGTON AVE TOLEDO OH 43699 MED COLL OHIO,DEPT IMMUNOL & MICROBIOL TOLEDO OH 43699 VET AFFAIRS MED CTR SAN DIEGO CA 92161
Titolo Testata:
Gene
fascicolo: 1-2, volume: 198, anno: 1997,
pagine: 387 - 391
SICI:
0378-1119(1997)198:1-2<387:IACOTU>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
JACK BEAN UREASE; SEQUENCE; CLONING; ANTIGEN;
Keywords:
FUNGAL RESPIRATORY PATHOGEN; GENOMIC/CDNA SEQUENCES; SOUTHERN/NORTHERN HYBRIDIZATION; PROTEIN EXPRESSION; PROTEIN HOMOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Yu et al., "ISOLATION AND CHARACTERIZATION OF THE UREASE GENE (URE) FROM THE PATHOGENIC FUNGUS COCCIDIOIDES-IMMITIS", Gene, 198(1-2), 1997, pp. 387-391

Abstract

The urease (URE)-encoding gene from Coccidioides immitis (Ci), a respiratory fungal pathogen of humans, was cloned, sequenced, chromosome-mapped and expressed. Both the genomic and cDNA sequences are reported. The transcription start point and poly(A)-addition site were confirmed. The URE gene contains eight introns and a 2517-bp ORF that translates a 839-amino-acid (aa) protein of 91.5 kDa and pi of 5.5, as deducedby computer analysis of the nucleotide sequence. The translated protein revealed eight putative N-glycosylation sites. The deduced URE showed comparable levels of homology to reported URE of the jack bean plant (Canavalia ensiformis; 71.8%) and URE of several genera of bacteria (Bp, 71.7%; Hp, 68.3%; Ka, 71.6%; Pm, 71.9%). The URE gene was mapped to chromosome III of Ci and was shown to be a single copy gene by Southern hybridization. Expression of a 1687-bp fragment of the URE gene in E. coli resulted in the production of a 63-kDa recombinant protein that was recognized in an immunoblot by antiserum raised against the KaURE homolog. This is the first report of a fungal URE gene. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/06/18 alle ore 17:06:24